Tm Reference Matrix The row denotes 1st amino acid, and the column denotes 2nd amino acid.
1\2	A	C	D	E	F	G	H	I	K	L	M	N	P	Q	R	S	T	V	W	Y
A	100	100	48.5960346	41.7977528	100	100	100	100	126.0671	129.5372051	150.0164204	58.77509007	100	36.02315288	54.3698565	100	141.1021937	167.790262	100	86.9914594
C	28.2147316	100	-78.0249167	29.3333333	100	161.4134276	100	32.07865169	100	100	100	100	255.1955307	100	100	100	-62.0952381	100	100	100
D	100	100	100	100	142.1040724	-12.0777662	24.7453954	55.84614809	100	134.1185483	25.0989011	52.44546498	100	164.663585	100	142.768015	48.00336276	139.755245	-99.0004346	100
E	100	26.68224299	100	109.791362	100	178.1683036	100	100	100	64.40677966	101.6030569	143.3672758	62.6063802	54.54545455	142.094785	83.1481648	115.1290683	100	100	63.52915
F	100	167.9411765	100	100	3.448627691	100	248.363712	87.22926456	153.083578	16.66033846	100	100	3.448627691	168.4113866	100	143.94958	100	26.2626263	196.8060662	100
G	100	100	100	174.4	107.3376752	124.0544894	100	149.6248064	66.464135	105.4107419	51.88288743	137.924812	100	-15.2530919	61.3333333	138.354366	100	2.2	151.3250883	67.2555948
H	100	402.1126761	93.0147371	100	177.4026512	100	100	-58.8623574	24.4941732	33.41404358	100	36.01009039	188.7009206	100	28.5523794	183.822938	100	100	100	100
I	100	-43.0022075	35.2527407	100	-37.3786306	136.2119205	243.978478	100	100	100	100	100	100	100	100	100	157.7638607	100	100	152.755484
K	100	28.98477157	100	100	105.2914691	100	100	100	141.245587	47.4719302	100	165.0772501	58.29339534	100	136.990155	57.5237504	100	131.748962	100	120.531026
L	100	56.34868421	100	131.655804	100	63.71582667	100	100	100	133.45741	32.19924812	100	100	54.23728814	100	100	100	100	100	172.78976
M	164.45822	100	225.89011	23.4482759	100	152.5517241	100	100	100	100	-209.036861	219.1471215	100	100	100	46.6122449	-37.2413793	37.0779221	100	100
N	100	-75.8897243	100	101.992004	100	100	100	100	3.49133658	100	164.3246046	-2.24998587	100	116.4353653	166.413495	100	100	161.203008	100	88.0850595
P	153.997303	159.4972067	100	100	100	61.01010101	100	100	100	115.8442037	100	100	139.0031522	168.4113866	33.9757363	100	52.29416613	88.6338243	100	100
Q	100	-8.48484848	146.995378	156.363636	42.05693297	132.4094523	188.490317	60.14747191	100	95.10899866	100	100	100	252.6586107	15.8376479	63.7276786	100	43.6363636	-40.5594406	148.697917
R	62.1369788	100	139.736347	66.3108997	100	100	25.8503401	16.33554084	136.990155	100	100	100	158.5534363	47.51294379	143.94454	67.5739645	100	93.577192	100	100
S	68.757526	100	158.959707	68.611482	148.838074	100	88.5218762	100	100	135.6140168	100	100	100	100	100	44.6309609	100	55.6168831	100	100
T	129.112493	100	100	105.986211	100	100	100	100	34.1169379	100	100	100	62.75299936	100	100	100	100	100	-26.8131868	100
V	100	173.030303	100	100	100	73.3697398	24.4897959	100	173.68989	158.2031313	100	100	43.63636364	100	53.3333333	100	87.30813995	146.25	100	41.1976912
W	100	-152.820513	100	25.0146028	100	151.3250883	100	210.5161517	100	-69.1003911	305.8928571	100	-83.9248876	100	158.376479	25.4910714	100	100	100	-22.8340081
Y	100	100	150.900981	139.76413	100	11.70526316	191.482227	100	100	100	100	88.08505947	197.4727321	212.4255952	100	40.0516629	100	30.8982684	100	21.579743